Authors:

Bennett A, Bar-Zeev N, Jere KC, Tate JE, Parashar UD, Nakagomi O, Heyderman RS, French N, Iturriza-Gomara M, Cunliffe NA.

Abstract:

We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshold cycle value (26.7) that predicts rotavirus-attributable gastroenteritis in this population. These data will inform the evaluation of direct and indirect rotavirus vaccine effects in Africa.

Journal:

J Clin Microbiol.

Year:

2015

PMID:

25854480

PMCID:

PMC4432065

Hyperlink:

http://www.ncbi.nlm.nih.gov/pubmed/25854480